Coomassie brilliant blue

Coomassie brilliant blue is the name of two similar triphenylmethane dyes that were developed for use in the textile industry but are now commonly used for staining proteins in analytical biochemistry. Coomassie brilliant blue G-250 differs from Coomassie brilliant blue R-250 by the addition of two methyl groups. The name "Coomassie" is a registered trademark of Imperial Chemical Industries.

Coomassie Brilliant Blue R-250
Names
Other names
C.I. 42660, C.I. Acid Blue 83
Brilliant indocyanine 6B, Brillantindocyanin 6B
Brilliant Cyanine 6B, Serva Blue R
Identifiers
3D model (JSmol)
ECHA InfoCard 100.025.509
PubChem CID
UNII
SMILES
  • CCN(CC1=CC(=CC=C1)S(=O)(=O)O)C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC.[Na+]
Properties
C45H44N3NaO7S2 (Sodium salt)
Molar mass 825.97 g/mol
Insoluble in cold, slightly soluble in hot (bright red blue)
Solubility in ethanol Slightly soluble
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references
Coomassie brilliant blue G-250
Solid Coomassie brilliant blue G
Brilliant blue G in isopropanol solution
Names
Other names
C.I. 42655, C.I. Acid Blue 90
Brilliant indocyanine G, Brillantindocyanin G
Xylene Brilliant Cyanine G, Serva Blue G
Identifiers
3D model (JSmol)
ECHA InfoCard 100.025.509
KEGG
PubChem CID
SMILES
  • CCN(CC1=CC(=CC=C1)S(=O)(=O)O)C2=CC(=C(C=C2)/C(=C\3/C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)O)C=C3C)/C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC)C
Properties
C47H50N3NaO7S2 (Sodium salt)
Molar mass 856.03 g/mol
Slightly soluble in cold, soluble in hot (bright blue)
Solubility in ethanol Soluble
Pharmacology
Legal status
  • CA: ℞-only
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references
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