Bis-tris methane

Bis-tris methane, also known as BIS-TRIS or BTM, is a buffering agent used in biochemistry. Bis-tris methane is an organic tertiary amine with labile protons having a pKa of 6.46 at 25 °C. It is an effective buffer between the pH 5.8 and 7.2. Bis-tris methane binds strongly to Cu and Pb ions as well as, weakly, to Mg, Ca, Mn, Co, Ni, Zn and Cd.

Bis-tris methane
Names
Preferred IUPAC name
2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
Other names
  • Bis-(2-hydroxy-ethyl)-amino-tris(hydroxymethyl)-methane
  • 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol
  • 2,2-Bis(hydroxymethyl)-2,2,2-nitrilotriethanol
Identifiers
3D model (JSmol)
2205275
ChEBI
ChemSpider
ECHA InfoCard 100.027.489
EC Number
  • 230-237-7
4519
MeSH Bistris
PubChem CID
UNII
  • InChI=1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2 Y
    Key: OWMVSZAMULFTJU-UHFFFAOYSA-N Y
SMILES
  • OCCN(CCO)C(CO)(CO)CO
Properties
C8H19NO5
Molar mass 209.242 g·mol−1
Appearance White crystals
Odor Odourless
Melting point 102 to 103 °C (216 to 217 °F; 375 to 376 K)
209.2 g L−1 (at 20 °C)
Acidity (pKa) 6.46
Basicity (pKb) 7.54
UV-vis (λmax) 280 nm
Absorbance 0.15
Hazards
GHS labelling:
Warning
H315, H319, H335
P261, P305+P351+P338
NFPA 704 (fire diamond)
2
0
0
Related compounds
Related alkanols
Related compounds
 Diethylhydroxylamine
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
N verify (what is YN ?)
Infobox references
This article is issued from Wikipedia. The text is licensed under Creative Commons - Attribution - Sharealike. Additional terms may apply for the media files.