Barnase
Barnase (a portmanteau of "BActerial" "RiboNucleASE") is a bacterial protein that consists of 110 amino acids and has ribonuclease activity. It is synthesized and secreted by the bacterium Bacillus amyloliquefaciens, but is lethal to the cell when expressed without its inhibitor barstar. The inhibitor binds to and occludes the ribonuclease active site, preventing barnase from damaging the cell's RNA after it has been synthesized but before it has been secreted. The barnase/barstar complex is noted for its extraordinarily tight protein-protein binding, with an on-rate of 108s−1M−1.
| Barnase | |||||||
|---|---|---|---|---|---|---|---|
The tightly bound complex between barnase and its inhibitor barstar. Barnase is colored by secondary structure and barstar is colored in blue. | |||||||
| Identifiers | |||||||
| Symbol | Barnase | ||||||
| PDB | 1BRS More structures | ||||||
| UniProt | P00648 | ||||||
| Other data | |||||||
| EC number | 3.1.27.- | ||||||
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