1965 Australian Championships – Women's singles

First-seeded Margaret Smith was the five-time defending champion, and successfully defended her title, defeating Maria Bueno 5–7, 6–4, 5–2 ret. in the final to win the women's singles tennis title at the 1965 Australian Championships.

Women's singles
1965 Australian Championships
Champion Margaret Smith
Runner-up Maria Bueno
Score5–7, 6–4, 5–2 ret.
Draw52
Seeds16
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